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Nanoparticles presenting biomimetic ligands influence mineralization of calcium phosphate | Poster Board #1408

Date
March 26, 2023

Gold nanoparticles with a variety of ligands were found to act as biomimetic templates for the mineralization of calcium phosphate. Gold nanoparticles are useful scaffold for the development of multi-functional materials due to their ease of synthesize and functionalization. This work describes the synthesis, characterization, and analysis of these gold nanoparticles, as well as methods of attaching known DNA aptamers to their surface. Nanoparticles were synthesized with a range of ligands, including glutathione (GSH), tiopronin (TIO), 3-mercaptobenzoic acid (3MBA), and poly-ethylene glycol (PEG). TEM analysis revealed approximately 4 nm diameter gold cores depending on the ligand and the synthesis method. DNA linker and aptamers were attached through place exchange and base-pair hybridization, purified through spin filtration, and analyzed with absorbance. Nanoparticles presenting both GSH and PEG were found to promote mineralization at a higher degree than those with TIO or 3MBA at concentrations of 12.5 nM and 25 nM. Mineralization results for the DNA aptamer-presenting nanoparticles are in progress with the goal of creating a nanoparticle library for biomimetic mineralization templates.

Presenter

Speaker

Speaker Image for Aren Gerdon
Associate Professor, Emmanuel College

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