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3825763
DNA origami nanostructures as biomimetic scaffolds for calcium phosphate mineralization | Poster Board #501
Date
March 27, 2023
Collagen fibrils play an important role in the formation of bone as the structural component for the mineralization of calcium phosphate. DNA origami nanostructures are explored in this work as a potential substitute for the role of collagen in the formation of bone by analyzing the viability of these nanostructures as biomimetic scaffolds for calcium phosphate mineralization. A DNA origami tile nanostructure was assembled and then characterized through gel electrophoresis, dynamic light scattering, and atomic force microscopy (AFM). A DNA aptamer previously identified to promote calcium phosphate mineralization was also attached to the tile's surface. Calcium phosphate mineralization in the presence of the DNA origami nanostructures was analyzed in solution using absorbance measurements and on a surface using AFM. The DNA origami nanostructures were found to positively influence calcium phosphate mineralization compared to controls. Current efforts focus on further observing the influence of the DNA origami nanostructures with and without the DNA aptamer in mineralization as well as understanding the interaction between the origami and mineral morphology and crystallinity.
Collagen is a known biological structural scaffold that is important in the calcium phosphate mineralization of bone. Real-time, kinetic analysis of collagen mineralization has not previously been reported with good time resolution…
Instructors in chemistry are motivated to deliver content and improve student understanding of the physical and chemical world. Doing this while ignoring student’s personal connections, experiences, and emotions – positive or negative - may undermine the educational goal…