4198363

Scalable purification with prepacked ceramic hydroxyapatite process-scale chromatography columns | Poster Board #122

Date
March 25, 2025

We evaluated the purification profiles of the following ceramic hydroxyapatite prepacked columns: 1.6 cm, 8 cm, 33 cm, and 45 cm inner diameter (ID) with 20 cm bed height. Each column used in the study was prepacked with CHT™ Ceramic Hydroxyapatite Type I Media (40 μm particle size). A sample mixture containing bovine serum albumin, lysozyme, and lactoferrin was loaded onto the column. A buffer of 5 mM sodium phosphate and 10 mM NaCl, pH 7.2, was used for equilibration. The sample mixture was then eluted with a linear gradient of 5 mM sodium phosphate, 10 mM NaCl, pH 7.2, to 400 mM sodium phosphate, pH 7.2.

We evaluated the purification profiles across all four columns to confirm the success of the method transfer and the scaled approach. The consistent purification profile results of the purification runs show that their peaks overlay from bench scale to the process-scale runs. This indicates that the Foresight Pro Column design and CHT Media packing methodology are optimized, delivering consistent results across the prepacked CHT Foresight Pro column sizes.

The use of traditional small-scale development techniques, combined with Foresight Pro prepacked columns, enables the research, process development, technical transfer, and manufacturing teams to efficiently achieve their objectives. This approach facilitates faster scaling techniques that require less material and minimizes risk. Prepacked chromatography columns offer several advantages including a reduction in buffers, resin usage, equipment footprints, labor costs, and increased flexibility in production schedule management.

Related Products

Thumbnail for Exploring the potential of chromatography resins in therapeutic treatment delivery | Poster Board #129
Exploring the potential of chromatography resins in therapeutic treatment delivery | Poster Board #129
Chromatography resins are commonly utilized in the purification and analysis of biomolecules for therapeutic purposes because they can produce high-yield, high-purity end products…
Thumbnail for Design of experiment approach for mAb purification process development using mixed-mode resins | Poster Board #3914
Design of experiment approach for mAb purification process development using mixed-mode resins | Poster Board #3914
A monoclonal antibody (mAb) purification process typically consists of three chromatography steps: a capture chromatography step followed by intermediate and polishing steps using various ion exchange resins and hydrophobic interaction chromatography resins…
Thumbnail for GMP ready, process-scale prepacked columns for high performance and stability in process-scale purification | Poster Board #4020
GMP ready, process-scale prepacked columns for high performance and stability in process-scale purification | Poster Board #4020
Process-scale prepacked columns are designed to be used for biopharmaceutical manufacturing applications…
Thumbnail for Purification of single-stranded oligonucleotides using anion exchange chromatography
Purification of single-stranded oligonucleotides using anion exchange chromatography
The production of pure single-stranded oligonucleotides requires efficient, scalable and cost-effective chromatographic purification methods applied after chemical synthesis. Key impurities in crude samples are length-based and are best removed by anion exchange chromatography…