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Investigations into the DNA-binding mode of doxorubicinone | Poster Board #1155
Date
March 25, 2025
Cancer treatment is one of the major challenges facing the modern biomedical profession. Development of new small-molecule chemotherapeutics requires an understanding of the mechanism of action for these treatments, as well as the structure–activity relationship. Study of the well-known DNA-intercalating agent, doxorubicin, and its aglycone, doxorubicinone, was undertaken using a variety of spectroscopic and calorimetric techniques. It was found that, despite conservation of the planar, aromatic portion of doxorubicin, the agylcone does not intercalate; it instead likely binds to the DNA minor-groove. Data from stopped-flow kinetics, the osmotic stress method, melting point studies, circular dichroism spectroscopy, and Van't Hoff thermodynamic analysis will be shared to support this conclusion.
Presenter
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