3923089

Improved DNA sensing resolution using highly crystalline monolayer molybdenum disulfide nanopores

Date
August 14, 2023
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Solid-state nanopores are promising tools for the detection of tiny biomolecules, such as DNAs, RNAs and proteins. Compared to biological nanopores, solid-state nanopores offer the advantages of robust mechanical properties, high chemical stability, and adjustable pore geometries. However, a major challenge for solid-state nanopores is the insufficient resolution for DNA molecule sequencing. To achieve high-resolution DNA sequencing, nanopores with diameters close to the size of DNA molecules are required. Conventional nanopore milling on monolayer molybdenum disulfide flakes under transmission electron microscopy for sub-5 nm pores has remaining issues that the shape of the nanopore can hardly be controlled as well as the loss of local crystallinity. In this study, we developed an improved nanopore drilling technique using ultrafine electron beams for monolayer molybdenum disulfide single crystals. We fabricated a 7 nm pore with high circularity at single nanometer resolution that the pore size and shape were precisely controlled. The sensing results of ssDNA oligonucleotides demonstrated a prolonged translocation time for each nucleotide (51 μs), approximately 20 times higher than that in polycrystalline molybdenum disulfide nanopores with a similar diameter reported in the literature, suggesting the importance of single crystallinity. This technique paves the way for the development of high-resolution solid-state nanopore biosensors for biomolecules.

Presenter

Speakers

Speaker Image for Hirofumi Daiguji
Professor, University of Tokyo

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