3821077

Activation of apoptotic caspase 3 by methylparaben, heptylparaben and mononitroparaben in human melanoma cells | Poster Board #321

Date
March 27, 2023

Parabens have been used as preservatives in personal care products for many years due to their lack of discoloration, odor, and taste properties when added to products. Their ability to maintain neutral pH and their antimicrobial characteristics prevent the growth of microorganisms and fungi. Parabens have enhanced personal care products to make them more appealing to customers through their preservative properties. While parabens have been found in everyday products for a number of years, controversies over their use have arisen as they have been linked to aversive effects and multiple skin cancers including malignant, melanoma, and contact eczema in the last several years. In this study, human M624nmelanoma cells were treated with 0.05 – 10mM paraben solution dissolved in ethanol or methanol and then added to complete DMEM for cell treatment. Clonogenic assays using crystal violet dye were used to analyze cell proliferation/death. Results from this study report the LC50 value of methylparaben (methyl 4-hydroxybenzoate) as well as two paraben derivatives heptylparaben and mononitroparaben. Apoptosis (programmed and controlled cell death resulting from cell damage) activation was also characterized through analysis of Caspase 3 protein activation using a colorimetric Caspase-3 microplate assay kit. Results indicate that toxicity and ability to promote apoptotsis depends on the chemical atructure of different parabens

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