4183179

Evaluation of anticancer effects by different fragments of amyloid-β on non-small cell lung cancer cells | Poster Board #384

Date
March 26, 2025

Alzheimer’s disease (AD), a neurodegenerative disorder, and cancer, a disease that occurs as a result of abnormal cell division, are two of the leading causes of death globally. Recently, these two diseases have been reported to share several key pathophysiological features and an inverse relationship between them exists; patients suffering from AD have some protection against developing tumors and a low chance of developing cancer. On the other hand, patients who have been diagnosed with cancer show a slower cognitive decline and are less likely to develop AD. Amyloid-β (Aβ), a 1-40/42 amino acid residue peptide that is generated as a result of the cleavage of the amyloid precursor protein, is likely partially responsible for this inverse relationship. The hydrophobic nature of the Aβ protein allows it to self-aggregate to form plaques in the brain of patients with AD, and this same oligomerization is detrimental to cancer cells. In addition, Aβ contains binding regions for acetylcholinesterase at residues 1-16 and 12-28. Previously, we reported that different fragments of Aβ along with the full length peptide, reduced the level of acetylcholine, which is known to enhance the growth of cancer cells, by increasing the activity of acetylcholinesterase in A549 lung cancer cells. In this study, the anticancer activities of several fragments, including 1-16, 1-28, 12-28, and 25-35, all synthesized by solid phase peptide synthesis, will be evaluated using an MTT assay and the IC50s of the peptide fragments will be reported.

Related Products

Thumbnail for Synthesis and evaluation of N- and C-terminal biotinylated peptides based on insulin-like growth factor binding protein-3 | Poster Board #1599
Synthesis and evaluation of N- and C-terminal biotinylated peptides based on insulin-like growth factor binding protein-3 | Poster Board #1599
IGFBP-3 (Insulin-like Growth Factor Binding Protein-3) is known to protect against lung cancer, inhibiting the survival and growth of non-small cell lung cancer A549 cells. The protein contains a C-terminal hyaluronan (HA) binding motif which may be linked to its anticancer effects…
Thumbnail for Current Topics in Biological Chemisry:
Current Topics in Biological Chemisry:
: [BIOL] Division of Biological Chemistry
Thumbnail for Current Topics in Biological Chemisry:
Current Topics in Biological Chemisry:
: [BIOL] Division of Biological Chemistry
Thumbnail for Serine-8 modification in the N-terminal fragment of amyloid β to investigate phosphorylation | Poster Board #382
Serine-8 modification in the N-terminal fragment of amyloid β to investigate phosphorylation | Poster Board #382
This study investigates the effect of phosphorylation of Ser8 in the N-terminal 1-16 fragment of the amyloid β peptide since this phosphorylation may be a mechanism for altering peptide shape and function and possibly influence cytotoxicity and aggregation…