3D printed microfluidic devices with porous polymer monoliths for preterm birth risk biomarker preconcentration and labeling

We are developing 3D printed microfluidics that offer rapid and automated determination of disease-linked biomarkers. We created a 3D printer that enables the construction of complex, integrated, microfluidic devices [1]. A key application of these 3D prints is in the measurement of maternal blood serum biomarkers that indicate the risk of a preterm birth. We use a combination of immunoaffinity extraction [2], solid phase extraction with on-column fluorescence labeling [3], and microchip electrophoresis [4]. Here, we show selective immunoaffinity monolith extraction and enrichment of multiple preterm birth risk biomarkers in 3D printed devices. We further demonstrate that these biomarkers can be preconcentrated and fluorescently labeled on reverse-phase monoliths formed in our 3D printed microfluidic systems. Finally, these processes of immunoaffinity extraction, preconcentration, fluorescent labeling and microchip electrophoresis are being integrated into 3D printed microfluidic devices. These assays and 3D printing procedures under development are highly promising in improving multiplexed biomolecular analysis.