Session: Posters:

2-Azabicyclo[2.2.1]hept-5-en-3-one (γ-lactam or Vince lactam) is an important precursor of carbocyclic nucleoside analogs, such as abacavir which is important chemotherapeutic agent in the treatment of human HIV and hepatitis B viruses. Traditionally, they are synthesized by chemical methods, but enzymatic synthesis of nucleosides can be more efficient and environmetally friendly. Gamma-Lactamase (EC 3.5.2) is a hydrolase that is utilized for the kinetic resolution of racemic γ-lactam to obtain gamma-lactam, the amino acid product from the resolution can also be used for preparation of candidates currently under clinical trials. In this study, we report a newly discovered lactamase to be integrated in the preparation of the antiviral drugs, followed by protein engineering to enhance the catalytic performance.

Dr Shuaihua Gao

Postdoc, UC Berkeley

Appearances