Over the past two decades, it has been frequently found that KIEs are temperature(T)-independent with a variety of wild-type enzymes but become T-dependent to different degrees for different variants…
It has been recently proposed that certain fast protein vibrations play a role in compressing reaction centers to close contacts for chemistry to occur. One way to study this physical origin of enzyme catalysis uses H-tunneling reactions that largely rely on the donor-acceptor distances (DADs)…