Session: General Posters:

O-Linked glycans are modifications that are typically performed in the endoplasmic reticulum and Golgi to alter the biological role of a protein. For example, O-linked fucose and glucose are both involved in Notch signaling; O-linked mannose is found in α-dystroglycan and is involved in interactions in extracellular matrix proteins; and O-linked galactose is involved in collagen fibril formation. Because of the known importance of these modifications, faster and less expensive routes to glycosylated amino acids in forms ready for solid-phase peptide synthesis (SPPS) could allow easier access to pure glycopeptides and defined structural analogs. Herein is reported an approach for the synthesis of a small library of O-linked glycosylated amino acids using automated flow processes. This approach addresses the long reaction times of previously reported syntheses of such targets and the high commercial cost of these building blocks. The resulting synthetic glycosylated amino acids can serve as analytical standards. An open-source Python-based software platform is used to automate these flow-based reactions, thus both the protocol and the experimental setup is easily transferable to other labs.

Nicola Pohl

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