Session: General Poster:

Simultaneous analytical method using LC/MSMS for imidazolinone herbicides from livestock (egg, milk, beef, pork and chicken) for monitoring was developed with QuEChERS preparation. Sample weighing (5 g) in a 50 mL conical tube, added 0.1 M potassium phosphate dibasic solution (5 mL) was shaken for 10 min. After 0.5 mL 6 N HCl, 10 mL acetonitrile were added, it was shaken for 10 min. And QuEChERS extraction salt (Original method, 4 g MgSO₄, 1 g NaCl) were added to the sample in the 50 mL conical tube. The mixture was strongly shaken for 1 min and was centrifuged at 3,000 g for 10 min. The acetonitrile layer was purification with dSPE (150 mg MgSO₄, 25 mg C18) and was centrifuged at 13,000 g for 5 min. The supernatant was filtered with a membrane filters (pore size: 0.2 um) before analysis.
ME (%, Matrix effect) for almost analytes range were -6.56 to 7.11%. MLOD (Method LOD) and MLOQ (Method LOQ) was calculated by S/N ratio. MLOQs were 0.01 mg/kg. The linear correlation coefficients (r²) were > 0.99 within the range of 2 ~ 100 ug/kg for all of the 7 imidazolinone herbicides. The percentages (of imidazolinone herbicides) recovers were in the range of 76.1 ~ 110.6% (0.01 mg/kg level), 89.2 ~ 100.7% (0.1 mg/kg level) and 94.4 ~ 104.4% (0.5 mg/kg level) within the validation criteria (recover; 70-120% with RSD < 20%)

Kyu-Won Hwang

Appearances