Division/Committee: [BIOT] Division of Biochemical Technology

Mammalian cell culture has become an essential means for production of an ever-increasing number of viral vaccines, recombinant proteins, monoclonal antibodies and other biopharmaceuticals. The advent of cell-based therapy and gene therapy, where the desired products are the cells (e.g., t-cells and stem cells) or viral capsids encoding the gene of interest, further expand the need for optimal cell culture conditions. The growth of mammalian cells in nutrient-enhanced media is profoundly intertwined with the cell’s metabolism and its response to the physicochemical environment. Over time, the field has matured into a multidisciplinary activity including genetic manipulation of cell lines to obtain highly productive clones, medium and feed development, bioreactor characterization, and process control to optimize conditions for desired productivity and product quality. This session will encompass multiple aspects of mammalian cell culture development, including advances in the development of improved production vectors, novel clone selection strategies, engineering of host cell lines, cell expansion strategies, metabolic pathway analysis and engineering, media development, multivariate analysis of processes and, innovative cell line and cell culture process development. Papers relevant to these topics are highly encouraged, but not limited to those focusing on cell line development, vector engineering, cell culture medium optimization, medium preparation and storage, medium chemistry understanding, medium impact on cell function and metabolism, effects of media in controlled environments, metabolic engineering, metabolic modeling, 'omics analyses of mammalian cells in varying culture conditions, and management of raw material changes in medium components.

Keith Tyo

Northwesten University

Appearances