Session: Chemistry of Materials: Metal Organic Frameworks:

Enzymes are highly efficient biocatalysts that participate in all essential biochemical reactions in living organisms. They have more recently been explored as green, eco-friendly alternatives for catalyzing industry-relevant chemical reactions. Enzyme-driven biocatalysts are advantageous in comparison to traditional catalysts because they typically create greener byproducts while still showing astonishing reaction rates with regio- and stereoselectivity of products; however, issues related to stability and storage have hampered their use in industry. Many enzymes are sensitive to even a small change in temperature, pH, or pressure, rendering them unstable in most industrial settings. This presentation will present results for a first generation of biocatalyst composites created using Metal-Organic Frameworks (MOFs) as solid supports for enzyme immobilizations in an effort to develop more stable, efficient biocatalysts. The co-immobilization of enzymes on amine-functionalized MOFs have shown enhanced functionality, stability, and recyclability compared with free enzymes in solution. In this work, different immobilization strategies of enzyme-MOF biocatalyst systems featuring glucose oxidase (GOx) and chloroperoxidase (CPO) with different MOFs will be reported. The success of each biocatalyst investigated was determined by monitoring stability and activity in solution using surface area analysis, powder X-ray diffraction and biochemical assays.

Kari Stone

Benedictine University

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