Session: Accurate Mass/High Resolution Mass Spectrometry for Environmental Monitoring & Remediation: Accurate Mass/High Resolution Mass Spectrometry for Environmental Monitoring & Remediation

The spiny redberry, Rhamnus crocea, is the single larval host plant to the rare Hermes copper butterfly, Lycaena hermes. Though R. crocea has a broad distribution throughout coastal California, Baja California and parts of Arizona, for unknown reasons L. hermes distribution only spans from San Diego county to northern Baja California. One theory for the sedentary behavior of L. hermes is that the leaf chemistry of R. croceadiffers in plants in and out of L. hermes range. Using an Agilent 6400 ESI MS, a novel flavone glycoside has been found within the leaf extracts of R. crocea. Currently, MS is being used to determine the fragmentation patterns of this compound and several other secondary metabolites. Once established, this information will be used to determine relative concentrations of these metabolites inside the range of the L. hermes versus outside of its range.

Prepared LCMS samples containing <i>Rhamnus crocea</i> leaf extracts.

Prepared LCMS samples containing Rhamnus crocea leaf extracts.

The novel flavone glycoside under investigation has a molecular weight of 740amu (appearing at 741m/z due to ESI).

The novel flavone glycoside under investigation has a molecular weight of 740amu (appearing at 741m/z due to ESI).

Jacqueline Trischman

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