Mango-waste residues exhibit complex structural compositions, consisting of a heterogeneous matrix of polysaccharides, cellulose and other biopolymers, which necessitate the production of multiple glycosidases for efficient breakdown. The selection of robust microbial strains capable of producing multiple hydrolytic enzymes, including cellulases and amylases, offers a promising approach for cost-effective bioprocessing. Thus, this study aimed at isolating glycosidase-producing bacterial and fungal decomposers of mango waste, an abundant fruit processing waste that poses disposal challenges. Bacteria and fungi including yeast were isolated and screened for enzyme activity using standard methods. Screening results showed that isolates possess dual amylolytic and cellulolytic activities desirable for commercial applications. Based on maximum specific enzyme activity, Bacillus subtilis and B. amyloliquefaciens were identified as potent amylolytic strains while B. licheniformis exhibited dual cellulolytic and amylolytic activity. Additionally, Aspergillus foetidus and A. tubingensis were selected as the most effective cellulase-producing fungi. The study demonstrated that media formulation, particularly the concentration of carbon and nitrogen sources, plays a crucial role in optimizing glycosidase production. Furthermore, enzyme activity was significantly influenced by temperature and pH, which directly affected catalytic efficiency. Maximum amylase activity for B. subtilis and B. amyloliquefaciens was obtained at 40 ^oC and pH 7.0 while B. licheniformis exhibited thermotolerant cellulase activity at higher temperature. The Aspergillus strains achieved optimal cellulase activity at 50 ^oC. These findings highlight the potential of these microbial strains for industrial cost-effective enzyme cocktail production and further optimization studies could enhance enzyme yields for biotechnological applications.

Felix Offei

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