Session: Analytical Division Poster:

The structure of cholesterol derivatives (3β,5α,6α)-cholestane-3,6-diol (cholestanediol) and 5-deutero-(3β,5α,6α)-cholestane-3,6-diol (D-cholestanediol), obtained by hydroboration-oxidation of (3β)-cholest-5-en-3-ol, was analyzed by NMR spectroscopy. Application of one- and two-dimensional NMR methods allowed partial determination of the crowded 1H and 13C spectra. Assignments for the 1H and 13C NMR spectra of cholesterol, (3β,5α,6α)-cholestane-3,6-diol, and 5-deutero-(3β,5α,6α)-cholestane-3,6-diol were made by using the following one- and two-dimensional NMR techniques: DEPT, 1D Proton, 1D, Carbon, 2D COSY, HMQC and HMBC.

Dr. David Martyn

Professor, Southwestern Oklahoma State University

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