Session: Advances in Electrochemistry:

The development of fast, cost-effective and robust analytical methods for disease-related biomarkers implementable into point of care testing (PoCT) devices results in a rapid and appropriate disease treatment and the consequent benefits for the patient. Myeloperoxidase (MPO) is a relevant biomarker overproduced in infection and inflammation events. The electrochemical methods previously presented for its quantification require tedious sample preparation and the use of complex protocols that are time consuming and increase the cost of the final PoCT device, thus limiting their use. The direct electrochemical quantification of MPO is hindered by the restricted accessibility to its active redox center, thus we take advantage of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as redox mediator to achieve an unblocked access to the active center. The herein developed method is based on the spontaneous oxidation of ABTS upon contact with MPO and the subsequent electrochemical reduction of the MPO-oxidized ABTS, being the charge involved in the latter process correlated with the concentration of MPO in the sample. This method permits the quantification of a wide range of MPO concentrations, including those considered relevant for diseases. Therefore, the developed simple and rapid electrochemical method represents a suitable analytical technique that could be implemented in PoCT devices for assessment of bacterial infection, chronic obstructive pulmonary disease or adverse cardiac events.

Arnau Bassegoda

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